The development and large-scale deployment of emerging biotherapy and biomedicine technologies encompass multiscale biological processes from molecular interactions to macroscopic bioreactor operations. Within this context, microfluidic technology serves as the cornerstone for achieving precise bioprocess control. To address the common demands of biosensing, bioediting, bioculture, and high-throughput screening, we have conducted innovative research spanning microfluidic actuation, sensing and detection, and intelligent control. Based on AI-assisted architecture, we established programmable microfluidic actuation methods that bridge continuous and discrete flow regimes, as well as multiscale control and sensing technologies ranging from single-molecule to single-cell levels. We also fully self-developed a series of novel instrument systems. Our findings have been published in over 20 high-impact journals, including Nature sub-journals. Two of the instrumental technologies have been successfully transferred for practical application and are now utilized by more than 120 universities and research institutes both in China and abroad.
